Reporter
Part:BBa_K3176001:Design
Designed by: Tara Fournier Group: iGEM19_Pasteur_Paris (2019-10-16)
mRFP for S.aureus with SarA RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 623
Illegal AgeI site found at 735 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
During the design of the sequence, we had to chose the right promoter in order to allows a great expression of mRFP1 and add the SarA RBS
Source
BBa_K3176001 was adapted from BBa_J04450 for the mRFP and BBa_K1323021 for the sarA P1 promoter